Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp

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Engineering production of functional scFv antibody in E. coli by co-expressing the molecule chaperone Skp

Single-chain variable fragment (scFv) is a class of engineered antibodies generated by the fusion of the heavy (VH) and light chains (VL) of immunoglobulins through a short polypeptide linker. ScFv play a critical role in therapy and diagnosis of human diseases, and may in fact also be developed into a potential diagnostic and/or therapeutic agent. However, the fact that current scFv antibodies...

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ژورنال

عنوان ژورنال: Frontiers in Cellular and Infection Microbiology

سال: 2013

ISSN: 2235-2988

DOI: 10.3389/fcimb.2013.00072